FACILITIES & EQUIPMENT AVAILABLE
Recombinant protein expression and purification:
Bacterial culture room with orbital shakers, incubators, hoods; BioFlo 110 Biofermentor (New Brunswick Scientific), Ultrasonic Homogenizers; ÄKTA Purifier FPLC system (GE Healthcare);
Mammalian cell cultures:
Several mammalian cell lines, including HeLa (cervical adenocarcinoma, both wild type and inducible Ape1 knock-in/knock-out), NPM1+/+ or NPM1-/- MEFs, OCI-AML2 and 3 (acute myeloid leukemia cell line, either wild type or expressing mutant NPM1c+, respectively), HUH7 and JHH6 (hepatocarcinoma cell lines displaying different differentiation phenotype and Ape1 and NPM1 expression levels); SK-OV-3 (ovarian cancer), SHSY5Y (neuroblastoma), SAOS-2 (p53-null), U2OS (p53-intact), HCT116 p53+/+ and isogenic HCT116 p53-/-, etc.; mammalian cell cultures room with hoods, incubators and centrifuges;
Microscopy facilities:
DM IRBE - TCS SP Laser Scanning Confocal microscope (Leica); Live-cell imaging station equipped with an epifluorescence DMI6000 B microscope (Leica);
Molecular imaging instrumentations:
Odyssey CLx imager for fluorescence NIR spectroscopy (LI-COR);
Proteomics facility:
Ettan IPGphor II isoelectric focusing systems, Hoefer SE 600 Ruby vertical electrophoresis systems (GE Healthcare), Image Scanner UMAX (Amersham Biosciences) and Image Master 2D Platinum 6.0 (GE Healthcare); ChemiDoc XRS for Western blotting detection (Biorad); Mass spectrometry facility with LC/MS LCQXPDECA Ion Trap Mass Spectrometer;LC/MS System Single quadrupole MSQ; Biacore 2000 and Biacore X-100 Surface Plasmon Resonance Systems (Biacore);
Multipurpose equipment:
Ultrospec 1100 Pro Spectrophotometer (GE Healthcare); Nanodrop 2000 (Thermo Scientific); Centrifuges: Megafuge 1.0 R (Heraeus), RC-5B and Evolution RC (Sorvall), 5810 R (Eppendorf), Ultracentrifuge Centrikon T-1055 (Kontron), two top bench centrifuges; iQ5 Multicolor and CFX96 Real-Time Detection Systems (Biorad); FACS Scan (Becton Dickinson)