MAIN TECHNIQUES
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Expression and purification of recombinant proteins in E. coli: biofermentor (BioFlo110), chromatographic purification through FLPC (Akta Prime)
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Characterization of kinetics and thermodynamics parameters through biophysical techniques: circular dichroism, surface plasmon resonance, UV spectroscopy
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Gene expression profiles by microarray and qPCR analyses
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Molecular biology techniques: gene cloning, mutagenesis, enzymatic assays, plasmid manipulation, gene silencing techniques, EMSA, pulse-chase experiments
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Biochemical techniques: Western blotting, SDS-PAGE, chromatography, ELISA
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Cell biology techniques: cell cultures, cell viability and proliferation assays, generation of stable and inducible gene ki/kd cell lines
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Gene studies: ChIP and RIP analyses
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Proteomics and interactomics techniques: 2-DE analysIs, co-immunoprecipitation and GST-pull down
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Confocal microscopy and molecular imaging: Immunofluorescence assays, Proximity Ligation Analysis (PLA), live confocal analysis.
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Drug screening /development: alpha screen technology, li-cor technology
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Organoids development from colon cancer